Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 28, 2016; 22(48): 10584-10591
Published online Dec 28, 2016. doi: 10.3748/wjg.v22.i48.10584
Regulating effect of TongXie-YaoFang on colonic epithelial secretion via Cl- and HCO3- channel
Cheng Yang, Ying Xiong, Sheng-Sheng Zhang, Fang-Mei An, Jing Sun, Qing-Lin Zhang, Qiang Zhan
Cheng Yang, Fang-Mei An, Jing Sun, Qing-Lin Zhang, Qiang Zhan, Department of Gastroenterology, Wuxi People’s Hospital, Nanjing Medical University, Wuxi 214023, Jiangsu Province, China
Ying Xiong, Community Health Service Center of Jinkui Street, Wuxi 214023, Jiangsu Province, China
Sheng-Sheng Zhang, Digestive Disease Diagnosis and Treatment Center, Beijing Chinese Medicine Hospital, Capital Medical University, Beijing 100010, China
Author contributions: Yang C made contributions to the study design, data interpretation, and wrote the manuscript; Zhang SS made contributions to the study design; Xiong Y, An FM, Sun J and Zhan Q performed the majority of experiments and data analysis; Zhang QL conducted the majority of quality control for the herbs of the formula.
Supported by Medical and public health technology research and development projects of WuXi science and technology development fund, No. CSE31N1501.
Institutional review board statement: All experiments were reviewed and approved by the Ethical Committee and Institutional Animal Care and Use Committee of Dongzhimen Hospital, No. SYXK(Beijing)2009.0028, and the methods were performed in strict accordance with the Animal Management Rules of the Chinese Ministry of Health.
Institutional animal care and use committee statement: All animal care and experimental procedures were conducted according to the institutional ethical guidelines and conformed to the requirements of the Institutional Animal Care and Use Committee of Beijing University of Chinese Medicine and the Animal Ethics Committee of Dongzhimen Hospital, No. SYXK(Beijing)2009.0028.
Conflict-of-interest statement: No potential conflicts of interest relevant to this article were reported.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Zhan Qiang, PhD, Associate Professor, Department of Gastroenterology, Wuxi People’s Hospital, Nanjing Medical University, 299 Qing-Yang Road, Nan-Chang District, Wuxi 214023, Jiangsu Province, China.
Telephone: +86-510-85351120 Fax: +86-510-85351120
Received: August 23, 2016
Peer-review started: August 24, 2016
First decision: September 28, 2016
Revised: October 10, 2016
Accepted: November 15, 2016
Article in press: November 16, 2016
Published online: December 28, 2016
Core Tip

Core tip: Diarrhea-predominant irritable bowel syndrome (D-IBS) is a chronic functional gastrointestinal disease. Abnormal ion secretion of colonic mucosal epithelial cells is recognized as one of the pathophysiological factors. In this paper, through the observation of TongXie-YaoFang (TXYF) formula, a Chinese herbal formula, to D-IBS rats obtained by neonatal maternal separation plus restraint stress. The mucosal stripping under a microscope was used for tissue preparation. Short-circuit current technology was used for testing 5-HT-induced changes in the current across ion channels of colonic epithelium. The results indicated that TXYF-formula could regulate the secretion of Cl- and HCO3- in colonic mucosa via cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, Cl-/HCO3- exchanger, Na+-HCO3- co-transporter and Na+-K+-2Cl- co-transporter co-transporters.