Silencing Bmi-1 enhances the senescence and decreases the metastasis of human gastric cancer cells

Figure 1 Annealing of siRNA hairpin DNA by electrophoresis. M: DNA marker; 1: Hairpin single-stranded DNA products for 1104F and 1104R; 2: Hairpin single-stranded DNA product for 1356F and1356R.

Figure 2 DNA sequence of the inserted fragment by transfected bacteria recombinant plasmid. A: The DNA sequence of the inserted fragment by recombinant plasmid pRNAT-U6.2-si1104. B: The DNA sequence of the inserted fragment by recombinant plasmid pRNAT-U6.2-si1356. The two DNA sequences of the inserted fragment by recombinant plasmids corresponded to the designed sequences.

Figure 3 Levels of Bmi-1 mRNA and protein. A: The Bmi-1 mRNA level decreased in transfected BGC823 cells with pRNAT-U6.2-si1104 and pRNAT-U6.2-si1356, especially in pRNAT-U6.2-si1104 transfected BGC823 cells. B: The levels of Bmi-1 protein was higher in non-transfected and transfected BGC823 cells with empty vector pRNAT-U6.2, compared with transfected BGC823 cells targeting Bmi-1 (pRNAT-U6.2-si1104 and pRNAT-U6.2 -si1356). There was no expression in the transfected BGC823 cells with pRNAT-U6.2-si1104 targeting Bmi-1. 1: Transfected BGC823 cells with pRNAT-U6.2-si1104; 2: Transfected BGC823 cells with pRNAT-U6.2-si1356; 3: Transfected BGC823 cells with pRNAT-U6.2 (empty vector); 4: Non-transfected BGC823cells (blank).