Establishment of an orthotopic transplantation tumor model of hepatocellular carcinoma in mice

Figure 1 Representative images of fluorescence signals in nude mice detected by the International Veterinary Information Service system. The fluorescence signal intensity shows xenograft tumor size.

Figure 2 Harvesting orthotopic liver transplantation tumors. Tumors were harvested from the mice in groups II and III. Tumor size and weight in the paired box gene 5 (PAX5) group were smaller than those in the control group (P < 0.0001).

Figure 3 Tumor growth curve determined by the International Veterinary Information Service system. The P value was calculated using repeated measures analysis of variance. PAX5: Paired box gene 5.

Figure 4 Liver tissues from nude mice were detected by hematoxylin and eosin staining. A: Liver tumor, × 200; B: Normal liver, × 200.

Figure 5 Gene expression of paired box gene 5 in liver tumor tissues of mice in situ which was detected by reverse transcription-polymerase chain reaction. The expression of β-actin which acted as the internal control was determined. PAX5: Paired box gene 5.