Quasispecies dynamics in main core epitopes of hepatitis B virus by ultra-deep-pyrosequencing

Figure 1 Variability of all codons analyzed from the 3 sequentially studied samples, corresponding to patient 4. Percentages higher than 2% are indicated at the top of the bars. 1: Master aminoacid different between treatment free and lamivudine samples.

Figure 2 Evolution of the most variable codons of the core region analyzed in the sequentially studied patient. The variable position and its corresponding master amino acid are shown on the X-axis. The main mutated amino acids are indicated at the top of the bars.

Figure 3 Quasispecies composition of the hepatitis B virus core region in the 3 sequentially analyzed samples. A: Baseline variants (1-15) in percentages ≥ 1%; B: Treatment-free variability; C: Lamivudine sample variability of the most frequent amino acid substitutions defined in Figure 2. Linkage analysis was also performed attending these most variable codons.