Effects of blueberry on hepatic fibrosis and transcription factor Nrf2 in rats

Figure 1 Liver tissue in each group of rats (HE staining, × 40 magnification). A: Light microscopy showing normal liver tissue in the control group; B: Marked fibrosis in the model group; C-E: Pathological change in the treatment group was milder compared with that in the model group.

Figure 2 Expression of Nrf2 protein in rat livers (immunohistochemical, × 400 magnification, arrows indicate cytolymph/nucleus-positive cells). A: Cytolymph-positive cells in the control group; B-E: More cytolymph/nucleus-positive cells in the model group and each treatment group compared with the control group.

Figure 3 Expression of Nqo1 protein in rat livers (immunohistochemical, × 400 magnification, arrows indicate cytolymph-positive cells). A: Few cytolymph-positive cells in the control group; B-E: More cytolymph-positive cells in the model group and each treatment group compared with the control group.

Figure 4 Expression of Nrf2 (A) and Nqo1 (B) protein assessed by Western blotting. Lane 1: Control group; Lane 2: Model group; Lane 3: Blueberry prevention group; Lane 4: DSHX prevention group; Lane 5: Blueberry + DSHX prevention group. The molecular weight of Nrf2 is 57 kDa, the molecular weight of Nqo1 is 31 kDa.