Brief Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Jul 7, 2009; 15(25): 3161-3165
Published online Jul 7, 2009. doi: 10.3748/wjg.15.3161
Figure 1
Figure 1 RFLP analysis of 23S rRNA to detect the A2142G mutation using MboII restriction enzyme. Lane 1: 100 bp DNA ladder; Lane 2: Digestion product of resistant isolate with A2142G mutation, showing two fragments of 662 and 476 bp; Lanes 3 and 4: Digestion products of two clarithromycin-resistant isolates with A2143G mutation; Lane 5: Digestion product of a clarithromycin-sensitive isolate with T2182C mutation.
Figure 2
Figure 2 RFLP analysis of 23S rRNA to detect the A2143G using BsaI restriction enzyme. Lane 1: 100 bp DNA ladder; Lanes 2 and 3: Digestion products of two clarithromycin-resistant isolates with A2142G mutation; Lanes 4 and 5: Restriction analysis of resistant isolates with A2143G mutation, showing three fragments of sizes 666, 418 and 60 bp; Lanes 6-8: Digestion products of clarithromycin-sensitive isolates.