Detection of hMSH2 and hMLH1 mutations in Chinese hereditary non-polyposis colorectal cancer kindreds

Figure 1 DHPLC showing variant elution peaks (A) and normal elution peaks (B) in exon 12 of hMSH2 gene.

Figure 2 HPLC variants confirmed by direct sequencing displaying a single nucleotide substitution of c. 1808A→G in exon 12 of hMSH2 gene.

Figure 3 Pedigree tree of HNPCC 5 kindred. Co: Colon; Bl: Bladder; En: Endometrium.

Figure 4 DHPLC variants confirmed by direct sequencing revealing a single nucleotide substitution of c. 1882A→G in exon 16 of hMLH1 gene.

Figure 5 Pedigree tree of HNPCC 2 kindred. Co: Colon.