Esophageal Cancer
Copyright ©2008 The WJG Press and Baishideng.
World J Gastroenterol. Jan 14, 2008; 14(2): 203-210
Published online Jan 14, 2008. doi: 10.3748/wjg.14.203
Figure 1
Figure 1 Schematic representation of the TIMP3 promoter. The schematic was generated by downloading the TIMP3 CpG island genetic element from, using the Human March 2006 Freeze. The relative location of TIMP3 exon 1 is indicated. Each vertical line represents the location of a CpG. The three overlapping regions (R1, R2 and R3) amplified for methylation analysis are shown.
Figure 2
Figure 2 Expression and methylation of TIMP3 in cancer cell lines. The level of TIMP3 mRNA in cell lines treated with either 0 or 1 &mgr;mol/L aza-dC was determined by qRT-PCR and normalized using PBGD. Methylation was determined by melt curve analysis in three overlapping regions R1, R2 and R3, and is summarized.
Figure 3
Figure 3 Methylation of TIMP3 in esophageal cancer cell lines. The X axis is temperature, the Y axis is fluorescence dF/dT.
Figure 4
Figure 4 Methylation of TIMP3 in ESCC samples. The X axis is temperature, the Y axis is fluorescence dF/dT.
Figure 5
Figure 5 Expression of TIMP3 in esophageal tissues. A: Strong heterogeneous cytoplasmic expression of TIMP3 was limited to the basal layers in normal squamous mucosa; B: TIMP3 expression in unmethylated ESCC underlying normal squamous mucosa; C: Unmethylated ESCC showing strong heterogeneous staining; D: Methylated ESCC showing the region of reduced expression.