Methylation of TIMP3 in esophageal squamous cell carcinoma

doi:10.3748/wjg.14.203

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 14, Issue 2

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2826)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-5) series, Tables (1-3) series.

Item

Count

PDF

223

HTML

2326

Figures (1-5)

143

Tables (1-3)

134

Sum=2826

Jan 14, 2008 (publication date) through Apr 24, 2024

Times Cited of This Article

Times Cited (16)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Esophageal Cancer

World J Gastroenterol. Jan 14, 2008; 14(2): 203-210
Published online Jan 14, 2008. doi: 10.3748/wjg.14.203

Methylation of TIMP3 in esophageal squamous cell carcinoma

Eric Smith, Neville J De Young, Zi-Qiang Tian, Maria Caruso, Andrew R Ruszkiewicz, Jun-Feng Liu, Glyn G Jamieson, Paul A Drew

Eric Smith, Neville J De Young, Glyn G Jamieson, Discipline of Surgery, School of Medicine, Faculty of Health Sciences, The University of Adelaide, Royal Adelaide Hospital, South Australia 5005, Australia

Zi-Qiang Tian, Jun-Feng Liu, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China

Maria Caruso, Andrew R Ruszkiewicz, Division of Tissue Pathology, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia

Paul A Drew, School of Nursing and Midwifery, Flinders University, Bedford Park, South Australia 5042, Australia

Correspondence to: Eric Smith, Discipline of Surgery, School of Medicine, Faculty of Health Sciences, The University of Adelaide, Royal Adelaide Hospital, South Australia 5005, Australia. eric.smith@adelaide.edu.au

Telephone: +61-8-82222855

Fax: +61-8-82225896

Received: June 4, 2007
Revised: July 29, 2007
Published online: January 14, 2008

Abstract

AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.

METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2’-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.

RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.

CONCLUSION: Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China.

Keywords: Esophageal squamous cell carcinoma, Immunohistochemistry, Methylation, Tissue inhibitor of metalloproteinase 3