Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Jun 7, 2006; 12(21): 3373-3379
Published online Jun 7, 2006. doi: 10.3748/wjg.v12.i21.3373
Figure 1
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Figure 1 Construction sketch of pYMICL12.
Figure 2
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Figure 2 Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: pGEM-T/EcoRI/XhoI; 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.
Figure 3
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Figure 3 A: Identification of pYMICL12. 1: PCR product of D12D DNA; 2: DNA size marker DL2000; 3: pYMID12/EcoRI/XhoI; 4: DNA size marker λDNA/HindIII; 5: pYES2.0/EcoRI/XhoI. B: Restriction analysis of recombinant plasmids pEMICL12. 1: Plasmid pEMICL12; 2: PCR product of D12D; 3: pET21a/ EcoRI; 4: pMAGL12/ XhoI; 5: pEMICL12/ EcoRI+ XhoI; 6: λDNA/HindIII marker.
Figure 4
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Figure 4 Percentage of linoleic acid in transgenic yeast induced by different concentration of galactose. 1: 1% galactose; 2: 2% galactose; 3: 3% galactose; 4: 4% galactose.
Figure 5
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Figure 5 Percentage of linoleic acid in transgenic yeast induced for different durations. 1.12 h; 2. 24 h; 3.36 h; 4. 48 h.
Figure 6
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Figure 6 Percentage of linoleic acid in transgenic yeast induced at different temperature. 1: 15°C; 2: 20°C; 3: 25°C; 4: 30°C.
Figure 7
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Figure 7 GC analysis of the total fatty acid in transgenic yeast, INVSc1 (pYMICL12). A: Total fatty acid in transgenic yeast, INVSc1 (pYMICL12) after induction by 3% galactose at 15°C for 24 h; B: Total fatty acid in transgenic yeast, INVSc1 (pYES2.0) after induction by 3% galactose at 15°C for 24 h; C: Linoleic acid; D: Oleic acid.
Figure 8
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Figure 8 Northern blot of total RNA of transgenic yeast induced at different temperature. 1: 15°C; 2: 20°C; 3: 25°C; 4: 30°C.
Figure 9
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Figure 9 SDS-PAGE of membrane protein in transgenic E. coli.1.Total protein of BL21; 2: low mass marker; 3: Total protein of BL21/pMICL12; 4: Total soluble protein of BL21/pMICL12 after super-centrifugation; 5: Membrane protein of BL21/pMICL12.
Figure 10
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Figure 10 Gas chromatographic analysis of fatty acid methyl esters of lipids catalysed by membrane protein of E. coli in system in vitro. A: Fatty acid components catalysed by membrane protein of BL21/ pMICL12; B: Fatty acid components catalysed by membrane protein of BL21/ pET21a; C: Linoleic Acid; D: Oleic Acid.