Activation of STAT3 signaling in human stomach adenocarcinoma drug-resistant cell line and its relationship with expression of vascular endothelial growth factor

Figure 1 Western blot analysis of phospho-STAT3 in different cell lines. Lane 1: SGC7901 cell line; lane 2: SGC7901/R cell line.

Figure 2 EMSA analysis of STAT3 DNA-binding activity in different cell lines. Lane 1: Competitive probe; lane 2: SGC7901/R cell line; lane 3: SGC7901 cell line.

Figure 3 Identification of VEGF mRNA by RT-PCR in different cell lines. M: Ladder marker; lane 1: SGC7901 cell line; lane 2: SGC7901/R cell line.

Figure 4 Immunocytochemical staining of VEGF in two human stomach adenocarcinoma cell lines (DAB×400). A: parental cell line SGC7901; B: drug-resistant cell line SGC7901/R.