Effect of mutated IκBα transfection on multidrug resistance in hilar cholangiocarcinoma cell lines

Figure 1 Macrorestriction map of mutated IκBα Plasmi. Lane 1: Plasmid DNA; lane 2: Plasmid DNA cut by Xbal and HandIII; lane 3: DNA marker.

Figure 2 EMSA of QBC₉₃₉ and QBC₉₃₉HCVC +cells transfected with mutated IκBα plasmid. Lane 1: Control group; lane 2: QBC₉₃₉HCVC +cells without transfected with mutated IκBα; lane 3: QBC939 cells not transfected with mutated IκBα; lanes 4 and 5: QBC939HCVC + cells transfected with mutated IκBα; lane 6: QBC939 cells transfected with mutated IκBα.

Figure 3 Expression of MDR-1mRNA in the QBC939 cells transfected with mutated IκBα. M: DNA marker. Lane 1: The QBC₉₃₉HCVC+ cells transfected with mutated IκBα, lane 2: The QBC₉₃₉HCVC + cells not transfected with mutated IκBα; lane 3: The QBC₉₃₉ cells not transfected with mutated IκBα; lane 4: The QBC₉₃₉ cells transfected with mutated IκBα.

Figure 4 Expression intensity of P-GP protein in QBC939 and QBC939HCVC+ cells transfected with mutated IκBα. A: Expression of P-GP in the non-transfection Group, LSAB ×400; B: Expression of P-GP after transfection of mutated IκBα, LSAB ×400.