Pathological mechanisms of alcohol-induced hepatic portal hypertension in early stage fibrosis rat model

Figure 1 Levels of plasma HA, ColIV and LN in model and control groups ^bP<0. 01 vs control.

Figure 2 Mallory, Sirius Red and immunohistochemical staining of liver tissue. A: Mallory staining of normal rat liver tissue, ×200 B: Mallory staining of rat alcoholic fibrosis liver tissue, ×400 C: Sirius Red staining of normal rat liver tissue and polarization microscopy, ×200 D: Sirius Red staining of rat alcoholic fibrosis liver tissue and polarization microscopy, ×200 E: Immunohistochemical staining of the first antibody of ColIV of normal rat liver tissue, ×200 F: Immunohistochemical staining of the first antibody of ColIV rat alcoholic liver fibrosis tissue, ×200 G: Immunohistochemistry staining of the first antibody of LN in normal rat liver tissue, ×200 H: Immunohistochemical staining of the first antibody of LN in rat alcoholic liver fibrosis tissue, ×200.

Figure 3 Density of ColIV and LN in positive granule area ^bP<0. 01 vs control in model and co.

Figure 4 Comparison of MMP-2 and TIMP-1 protein expression in hepatic non-parenchymal cells between model and control groups^bP<0. 01, model group vs control group; ^cP<0.05, TIMP-1 vs MMP-2.