Fusion expression of Helicobacter pylori neutrophil-activating protein in E.coli

doi:10.3748/wjg.v11.i3.454

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Jan 21, 2005 (publication date) through Apr 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 21, 2005; 11(3): 454-456
Published online Jan 21, 2005. doi: 10.3748/wjg.v11.i3.454

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Figure 1 Restriction analysis of pMAL-c2x-napA. Lane 1: 1 kb DNA ladder; Lane 2: pMAL-c2x-napA digested by EcoRI; Lane 3: pMAL-c2x-napA digested by EcoRI and SalI; Lane 4: pMAL-c2x digested by EcoRI; Lane 5 HP-napA PCR fragment.

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Figure 2 SDS-PAGE analysis of rMBP-NAP. Lane 1: empty plasmid induced by IPTG; Lane 2: TB₁(pMAL-c2x-napA) induced by IPTG; Lane 3: lower molecular weight protein marker; Lane 4: affinity chromatography purified rMBP-NAP.

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Figure 3 Western blotting of purified rMBP-NAP.

Citation: Kang QZ, Duan GC, Fan QT, Xi YL. Fusion expression of Helicobacter pylori neutrophil-activating protein in E.coli. World J Gastroenterol 2005; 11(3): 454-456
URL: https://www.wjgnet.com/1007-9327/full/v11/i3/454.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i3.454