Mitochondrial DNA sequence analysis of two mouse hepatocarcinoma cell lines

Figure 1 Restriction patterns of amplified fragments. F: Hca-F cells; P: Hca-P cells; M: PCR standard marker. A: Restriction patterns of tRNA Met+Glu+Ile digested by Apa I, Alu I, BamH I, Rsa I and Stu I, respectively; B: Restriction patterns of D-loop digested by Xba I, Apa I, Rsa I, Stu I and Alu I, respectively; C: Restriction patterns of ND3 digested by Hae III, Dra I, Stu I, Rsa I and Alu I, respectively.

Figure 2 Variations identified in the D-loop (bases 15 950-91) of mitochondrial DNA from two types of hepatocarcinoma cell lines. Sequence and base number were from the complete mouse mtDNA sequence reported by Bibb et al. (Cell 1981; 26: 167-180 ). The G at nucleotide 16007 was mutated to A in two hepatocarcinoma cell lines and T at nucleotide 16268 was mutated to C in Hca-F cell line.