Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Apr 14, 2005; 11(14): 2101-2108
Published online Apr 14, 2005. doi: 10.3748/wjg.v11.i14.2101
Figure 1
Figure 1 Effective half-life in 32P colloids 7. 4 and 14.8 MBq injected group averaged 13 d.
Figure 2
Figure 2 Results of immuno-histochemistry assay of CD34 expression in Pc-3. A: Plenty of stained and densely arranged micro-vessels in tumor field (arrow); B: After medication the tumor cells loosely arranged in tumor field with scanty of micro-vessels stained or no micro-vessels (SABC ×400); PCNA expression in Pc-3. C: In control site, tumor cells were densely arranged with poly nuclei stained in deep brown yellow; D: In each of the dosed sites loosely arranged tumor cells merely with few nuclei stained in light brown yellow color (SABC ×400).
Figure 3
Figure 3 TEM shows the changes in Pc-3 tumor cell; A: Tumor cells (arrow) in control group grew vividly with large nuclei and some nucleoli (12×103×1. 2); B: Tumor cells (arrow) were damaged, only the contour of nucleus remained (6×103); C: A few remaining tumor cells arranged as a glandular lumen (arrow) with small nucleoli (8×103×1.2); D: At low dose point, well differentiated Pc-3 cell with small nucleoli, plenty of euchromatin, many long micro-villi on cell surface (arrow), glandular lumens interplaced between the cells having the tendency of exocrine formation (15×103×1.2).
Figure 4
Figure 4 Transmission electron microscopy of ILN A: Metastatic Pc-3 cells (arrow) in lymphatic tissue of control group (4×103); B: After 32P colloids irradiation apoptotic cells (arrow) in the connective tissue and endothelium of lymphatic sinuses (2. 5×103); C: Denatured tumor cells (arrow) after medication (5×103×1.2); D: ILN under light microscope: lymphatic tissue recovered to normal 4 wk after medication (HE ×400).
Figure 5
Figure 5 Results of TEM: A: Cell necrosis, breaking into debris (arrow) (10×103); B: Early apoptosis (arrow). Chromatin aggregated at periphery (10×103×1.2); C: Medium stage of apoptosis (arrow) showing nucleus shrunk and broken (10×103×1.2).