Oral attenuated Salmonella typhimurium vaccine against MG7-Ag mimotope of gastric cancer

Figure 1 Incorporation of the MG7 epitope cDNA into HBcAg gene by PCR. An about 600 bp fragment was obtained (1,2). Lane M: DNA marker (100-3000 bp); lanes 1-2: the PCR product.

Figure 2 Identification of fusion gene vaccine. Lane M: DNA marker (100-3000 bp); lanes 1,2: the digestion product.

Figure 3 Western blot of the expression product of MG7/HBcAg fusion gene. Lane M: Protein marker (M_r); lane 1: X4550 transformed with empty pYA3341; lanes 2,3: X4550 transformed with pYA3341-MG7/HBcAg (after induction of IPTG for 2 and 4 h).