Inhibitor RNA blocks the protein translation mediated by hepatitis C virus internal ribosome entry site in vivo

Figure 1 Stable expression of IRNA and mIRNA in HHCC cells. 1: DL2000 DNA marker; 2, 3: IRNA RT-PCR; 4: mIRNA RT–PCR; 5: IRNA PCR.

Figure 2 Inhibitory effect of IRNA on luciferase expression mediated by different mechanism.

Figure 3 Inhibitory effect of IRNA on HCV Core expression mediated by HCV IRES(A: 40 × 10 vs B: 25 × 10). A: HCV Core protein of pCMVNCRluc transfection group, B: HCV Core pro-tein of pcRz-IRNA and pCMVNCRluc cotransfection group.

Figure 4 Detection of HCV core protein in HHCC lines stably expressing pcHCV Cluc (25 × 10).

Figure 5 Interference of IRNA with HCV replicon translation.