Human endostatin gene transfer, either naked or with liposome, has the same inhibitory effect on growth of mouse liver tumor cells in vivo

Figure 1 Detection of the recombinant plasmid pVAX-sEN using EcoR I and Kpn I digestion. A: Marker15000; B: pVAX1 digested by EcoR I; C: Recombinant plasmid pVAX-sEN digested by EcoR I; D: Recombinant plasmid pVAX-sEN digested by EcoR I and Kpn I.

Figure 2 Comparison of in vivo transfection using different ratios of liposome and DNA in liposome-DNA complex and naked DNA.

Figure 3 Comparison of in vivo transfection using liposome complex (the ratio of liposome and DNA is 1:1) containing different doses of DNA.

Figure 4 Inhibitory effect of recombinant secretive endostatin on mouse hepatoma cells.

Figure 5 In vivo endostatin expression in peripheral blood of treated mice.

Figure 6 Inhibition of tumor neovascularization by injecting recombinant plasmid.