Establishment and assessment of two methods for quantitative detection of serum duck hepatitis B virus DNA

Figure 1 Detection of EcoR I-digested pBR325 and recovered long fragment by 10 g/L agarose gel electrophoresis. Lane 1: Molecular marker; lane 2: pBR325 digested with EcoR I; lane 3: Long fragment of pBR325 (DHBV DNA).

Figure 2 Sensitivity of alkaline phosphatase direct-labeled probe.

Figure 3 Detection of products of DHBV DNA positive stan-dard with qPCR by 20 g/L agarose gel electrophoresis. Lane l: Negative control; lanes 2-7: DHBV DNA positive standard (2.76 × 10⁶-2.76 × 10¹ copies/µL); lane 8: DNA marker.

Figure 4 Amplification index standard curves of DHBV DNA positive standard.

Figure 5 Concentration standard curve of DHBV DNA posi-tive standard.