Protective role of metallothionein (I/II) against pathological damage and apoptosis induced by dimethylarsinic acid

Figure 1 Typical HE staining. The bar is 100 μm. A, B: the lungs from control of MT^-/-and MT^+/+ mice; C, D: the lungs from 750 mg/kg DMAA group of MT^-/-and MT^+/+ mice. Arrows in-dicate atelectasis and hemorrhage.

Figure 2 Typical HE staining. The bar is 100 μm. A, B: the livers from control of MT^-/-and MT^+/+ mice; C, D: the livers from 750 mg/kg DMAA group of MT^-/-and MT^+/+ mice. The arrows indicate necrosis.

Figure 3 Typical HE staining. The bar is 100 μm. A, B: the kidneys from control of MT^-/-and MT^+/+ mice; C, D: the kid-neys from 750 mg/kg DMAA group of MT^-/-and MT^+/+ mice. The arrows indicate the swelling of glomerulus and the surrounding tubular tissue and urinary space compression.

Figure 4 Apoptosis in lungs of MT^+/+ and MT^-/- mice detected by TUNEL twenty-four hours after oral DMAA treatment. A: Typical apoptotic cells in alveolar area of MT^-/- mice at a dose of 188 mg/kg body weight. Brown staining indicates the apoptotic cells. The bar is 20 μm. B: Typical apoptotic cells in bronchial area of MT^-/- mice at a dose of 188 mg/kg body weight. Brown staining indicates apoptotic cells. The bar is 20 μm. C: AI in alveolar area. D: AI in bronchial area. All the values were expressed as ^-χ±s. ANOVA with subsequent post hoc’s test was performed for comparison of AI. ^a,bSignificant difference at P < 0.05, P < 0.01 when compared with the corresponding control group. ^c,dSignificant difference at P < 0.05, P < 0.01 when com-pared with the dose-matched MT^-/- mice group.

Figure 5 Apoptosis in livers of MT^+/+ and MT^-/- mice detected by TUNEL twenty-four hours after oral DMAA treatment. A: Typical apoptotic cells in the liver of MT^-/- mice at a dose of 188 mg/kg body weight. Brown staining indicates apoptotic cells. The bar is 20 μm. B. AI in the livers. All values were ex-pressed as ^-χ±s. ANOVA with subsequent post hoc’s test was performed for comparison of AI. ^{a, b}Significant difference at P < 0.05, P < 0.01 when compared with the corresponding control group. ^c,dSignificant difference at P < 0.05, P < 0.01 when com-pared with the dose-matched MT^-/- mice group.

Figure 6 MT concentration in livers of MT^+/+ mice detected by radio-immunoassay. All the values were expressed as ^-χ±s. ANOVA with subsequent post hoc’s test was performed for comparison of AI. ^bSignificant difference at P < 0.01 when com-pared with the corresponding control group.