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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 14, 2020; 26(6): 614-626
Published online Feb 14, 2020. doi: 10.3748/wjg.v26.i6.614
Published online Feb 14, 2020. doi: 10.3748/wjg.v26.i6.614
Characterizing the composition of intestinal microflora by 16S rRNA gene sequencing
Wen-Jia Wang, You-Lian Zhou, Jie He, Zhi-Qiang Feng, Long Zhang, Xiao-Bo Lai, Jun-Xiao Zhou, Hong Wang, Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, South China University of Technology, Guangzhou 510030, Guangdong Province, China
Author contributions: Wang WJ was responsible for sequencing and data analyses; Zhou YL and He J analyzed dates after sequencing; Feng ZQ, Zhang L and Lai XB collected specimens; Zhou JX contributed to the article and revised it; Wang H conceived the study and drafted the manuscript; Both authors approved the final version of the article.
Supported by Guangdong Provincial Department of Science and Technology , No. 2014A020212568 ; National Key Clinical Specialized Special Funds Programs of China , No. 2013544 .
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of the First People’s Hospital of Guangzhou.
Conflict-of-interest statement: The authors have no conflict of interest to declare.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Hong Wang, MD, PhD, Chief Physician, Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, South China University of Technology, No. 1 Panfu Road, Yuexiu District, Guangzhou 510030, Guangdong Province, China. wong.hong@163.com
Received: August 13, 2019
Peer-review started: August 13, 2019
First decision: October 14, 2019
Revised: December 25, 2019
Accepted: January 11, 2020
Article in press: January 11, 2020
Published online: February 14, 2020
Peer-review started: August 13, 2019
First decision: October 14, 2019
Revised: December 25, 2019
Accepted: January 11, 2020
Article in press: January 11, 2020
Published online: February 14, 2020
Core Tip
Core tip: Colorectal adenomas (CRAs), the most important pre-cancerous lesions in colorectal cancer, have a close relationship with intestinal microflora. However, the mechanism is not clear. Early detection of CRA can effectively reduce the morbidity and mortality of colorectal cancer. This study analyzes the composition and diversity of intestinal microflora in patients with CRA through 16s rDNA gene sequencing. It was proven that the diversity of intestinal microflora in patients with CRA decreased, and that an increase in the number of Halomonadaceae and Shewanella algae may be biomarkers of CRA, which provides a basis for future research.