Towards a standard diet-induced and biopsy-confirmed mouse model of non-alcoholic steatohepatitis: Impact of dietary fat source
Michelle L Boland, Taylor S Cohen, David E Tabor, Fiona Fernandes, Andrey Tovchigrechko, Paul Warrener, Bret R Sellman, James L Trevaskis, Cardiovascular, Renal and Metabolic Diseases, MedImmune, Gaithersburg, MD 20878, United States
Michelle L Boland, Denise Oró, Kirstine S Tølbøl, Sebastian T Thrane, Jens Christian Nielsen, Sanne S Veidal, Jacob Jelsing, Michael Feigh, Niels Vrang, Henrik H Hansen, Pharmacology, Gubra, Hørsholm DK-2970, Denmark
Author contributions: Boland ML, Cohen TS, Warrener P, Sellman BR, Feigh M, Vrang N, Trevaskis JL, and Hansen HH designed and coordinated the study; Boland ML, Oró D, Tølbøl KS, Thrane ST, Nielsen JC, Tabor DE, and Fernandes F performed the experiments, acquired and analyzed data; Boland ML, Cohen TS, Tabor DE, Fernandes F, Oró D, Tølbøl KS, Thrane ST, Nielsen JC, Tovchigrechko A, Veidal SS, Feigh M, Jelsing J, Vrang N, Trevaskis JL, and Hansen HH interpreted the data; Boland ML, Jelsing J, Trevaskis JL, and Hansen HH wrote the manuscript; all authors approved the final version of the article.
Supported by the Innovation Fund Denmark, Tølbøl KS, No. 5016-00168B.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board at MedImmune and Gubra.
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals (licence No. 2013-15-2934-00784, The Animal Experiments Inspectorate, Denmark; protocol no. MI-17-0005, The Institutional Animal Care and Use Committee at MedImmune, Gaitherburg, MD, United States).
Conflict-of-interest statement: Michelle L. Boland and James L. Trevaskis were previously employed by MedImmune, LLC. Taylor S. Cohen, David Tabor, Fiona Fernandes, Andrey Tovchigrechko, Paul Warrener, and Bret R. Sellman are employed by MedImmune LLC. All other authors have nothing to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and theuse is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Henrik H Hansen, PhD, Senior Scientist, Pharmacology, Gubra, Hørsholm Kongevej 11B, Hørsholm DK-2970, Denmark. firstname.lastname@example.org
Received: April 26, 2019
Peer-review started: April 26, 2019
First decision: May 24, 2019
Revised: June 28, 2019
Accepted: July 19, 2019
Article in press: July 19, 2019
Published online: September 7, 2019