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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 21, 2015; 21(7): 2040-2046
Published online Feb 21, 2015. doi: 10.3748/wjg.v21.i7.2040
Published online Feb 21, 2015. doi: 10.3748/wjg.v21.i7.2040
Depletion of the IKBKAP ortholog in zebrafish leads to hirschsprung disease-like phenotype
William Wai-Chun Cheng, Man-Ting So, Vincent Chi-Hang Lui, Paul Kwong-Hang Tam, Maria-Mercè Garcia-Barcelo, Department of Surgery, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Clara Sze-Man Tang, Department of Psychiatry, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Hong-Sheng Gui, Center for Genomic Sciences of the Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Vincent Chi-Hang Lui, Paul Kwong-Hang Tam, Maria-Mercè Garcia-Barcelo, Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Author contributions: Cheng WWC, So MT, Gui HS and Tang CSM performed the experiments; Cheng WWC, Tang CSM, Lui VCH, Tam PKH and Garcia-Barcelo MM conceived and designed the research and analysed the data; Cheng WWC wrote the manuscript; Lui VCH and Garcia-Barcelo MM revised the manuscript.
Supported by Small Project Funding, the University of Hong Kong, No. 201209176125 to Cheng WWC; Hong Kong Research Grants Council HKU No. 778610M to Tam PKH; Health and Medical Research Fund No. 01121326 to Lui VCH and The University of Hong Kong Genomics Strategic Research Theme.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Maria-Mercè Garcia-Barcelo, Associate Professor, Department of Surgery, Li Ka Shing Faculty of Medicine, the University of Hong Kong, 9/F Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong, China. mmgarcia@hku.hk
Telephone: +852-2-8315073 Fax: +852-2-8199623
Received: April 30, 2014
Peer-review started: May 2, 2014
First decision: May 29, 2014
Revised: August 1, 2014
Accepted: September 12, 2014
Article in press: September 16, 2014
Published online: February 21, 2015
Peer-review started: May 2, 2014
First decision: May 29, 2014
Revised: August 1, 2014
Accepted: September 12, 2014
Article in press: September 16, 2014
Published online: February 21, 2015
Core Tip
Core tip: To investigate the functional role of IKBKAP in enteric nervous system (ENS) development, we knocked down the zebrafish ortholog ikbkap using a translation blocking antisense morpholino. Loss of ikbkap caused aganglionosis and a reduced number of enteric neurons, indicating that IKBKAP is important for proper ENS development.