Published online Nov 14, 2015. doi: 10.3748/wjg.v21.i42.11954
Peer-review started: April 24, 2015
First decision: July 13, 2015
Revised: July 29, 2015
Accepted: September 14, 2015
Article in press: September 14, 2015
Published online: November 14, 2015
Core tip: The combination of quantitative detection of hepatitis B surface antigen (HBsAg) and hepatitis B virus (HBV) DNA can be used to classify individuals during the course of HBV infection and to monitor therapy. The most popular platforms for HBsAg detection are based on chemiluminescent microparticle immunoassay, while polymerase chain reaction based methods are widely used for HBV DNA assay. Recently, isothermal amplification and biosensors offered a lower cost and more rapid alternative for HBV quantification. This article reviews the most commonly used quantitative methods for HBV.