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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 28, 2015; 21(12): 3499-3508
Published online Mar 28, 2015. doi: 10.3748/wjg.v21.i12.3499
Published online Mar 28, 2015. doi: 10.3748/wjg.v21.i12.3499
Degradation of intestinal mRNA: A matter of treatment
Sabine Heumüller-Klug, Karin Kaiser, Elvira Wink, Cornelia Hagl, Lucas Wessel, Department of Pediatric Surgery Mannheim, Medical University of Heidelberg, 68167 Mannheim, Germany
Carsten Sticht, Medical Research Centre Mannheim, Medical University of Heidelberg, 68167 Mannheim, Germany
Karl-Herbert Schäfer, University of Applied Sciences Kaiserslautern, 66482 Zweibrücken, Germany
Author contributions: Heumüller-Klug S designed, performed, analysed experiments and wrote the paper; Sticht C analysed data and wrote the paper; Kaiser K and Wink E preformed research; Hagl C co-ordinated the collection of all human material; Wessel L provided financial support for this work; and Schäfer KH designed the study, analysed data and wrote the paper.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Karl-Herbert Schäfer, MD, Professor, Head of Enteric Nervous System Group, University of Applied Sciences Kaiserslautern, 66482 Zweibrücken, Germany. karl-herbert-schaefer@hs-kl.de
Telephone: +49-631-37245418 Fax: +49-631-37245305
Received: July 21, 2014
Peer-review started: July 22, 2014
First decision: August 27, 2014
Revised: September 29, 2014
Accepted: November 7, 2014
Article in press: November 11, 2014
Published online: March 28, 2015
Peer-review started: July 22, 2014
First decision: August 27, 2014
Revised: September 29, 2014
Accepted: November 7, 2014
Article in press: November 11, 2014
Published online: March 28, 2015
Core Tip
Core tip: The quality of RNA is crucial for an appropriate RNA analysis. Especially when working with human material, precious samples will often be used for different purposes and can therefore not be frozen immediately. Gut tissue is especially fragile and RNA degrades rapidly if not treated adequately. Under these aspects RNA degradation of different gut sections and gut wall layers regarding their treatment was investigated in this study. Storage, rinsing and preparation conditions are essential for RNA stability. Sufficient and permanent cooling as well as the removal of bacterial contamination leads to a reduced degradation in muscle tissue of the gut.