Published online Mar 28, 2015. doi: 10.3748/wjg.v21.i12.3499
Peer-review started: July 22, 2014
First decision: August 27, 2014
Revised: September 29, 2014
Accepted: November 7, 2014
Article in press: November 11, 2014
Published online: March 28, 2015
Core tip: The quality of RNA is crucial for an appropriate RNA analysis. Especially when working with human material, precious samples will often be used for different purposes and can therefore not be frozen immediately. Gut tissue is especially fragile and RNA degrades rapidly if not treated adequately. Under these aspects RNA degradation of different gut sections and gut wall layers regarding their treatment was investigated in this study. Storage, rinsing and preparation conditions are essential for RNA stability. Sufficient and permanent cooling as well as the removal of bacterial contamination leads to a reduced degradation in muscle tissue of the gut.