Copyright
©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 7, 2015; 21(1): 164-176
Published online Jan 7, 2015. doi: 10.3748/wjg.v21.i1.164
Published online Jan 7, 2015. doi: 10.3748/wjg.v21.i1.164
Establishment and characterization of cell lines from chromosomal instable colorectal cancer
Claudia Maletzki, Martin Randow, Michael Linnebacher, Section of Molecular Oncology and Immunotherapy, University of Rostock, D-18057 Rostock, Germany
Michael Gock, Ernst Klar, Department of General Surgery, University of Rostock, D-18057 Rostock, Germany
Maja Huehns, Friedrich Prall, Institute of Pathology, University of Rostock, D-18057 Rostock, Germany
Author contributions: Klar E critically revised the paper; Maletzki C and Gock M contributed equally to this work; Maletzki C and Linnebacher M designed the research; Maletzki C and Randow M performed the research; Huehns M and Prall F performed molecular pathology and histology; Maletzki C, Gock M, Randow M and Linnebacher M analyzed the data; and Maletzki C, Gock M and Linnebacher M wrote the paper.
Supported by German Cancer Aid, No. 108919
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Michael Linnebacher, PhD, Section of Molecular Oncology and Immunotherapy, University of Rostock, Schillingallee 35, D-18057 Rostock, Germany. michael.linnebacher@med.uni-rostock.de
Telephone: +49-381-4946013 Fax: +49-381-4946002
Received: May 22, 2014
Peer-review started: May 23, 2014
First decision: June 18, 2014
Revised: June 26, 2014
Accepted: July 24, 2014
Article in press: July 26, 2014
Published online: January 7, 2015
Peer-review started: May 23, 2014
First decision: June 18, 2014
Revised: June 26, 2014
Accepted: July 24, 2014
Article in press: July 26, 2014
Published online: January 7, 2015
Core Tip
Core tip: We ultra-low passage and well-characterized tumor models are considered the basis for modern preclinical research, but are still rare for colorectal carcinoma (CRC). Herein describe two novel ultra-low passage patient-derived CRC cell lines, HROC18 and HROC32, which were established both direct from patient material and after xenografting. We characterized these models according to phenotype, molecular, morphological, and growth characteristics, as well as by drug response profiles. These cell lines expand our comprehensive collection of tumor models, which in summary provide a useful instrument for basic and translational research. The models are available on request.