Cheng X, Gao XC, Wang JP, Yang XY, Wang Y, Li BS, Kang FB, Li HJ, Nan YM, Sun DX. Tricistronic hepatitis C virus subgenomic replicon expressing double transgenes. World J Gastroenterol 2014; 20(48): 18284-18295 [PMID: 25561795 DOI: 10.3748/wjg.v20.i48.18284]
Corresponding Author of This Article
Dian-Xing Sun, MD, Professor of Medicine, The Liver Disease Diagnosis and Treatment Center of PLA, Bethune International Peace Hospital, 398 West Zhongshan Road, Shijiazhuang 050082, Hebei Province, China. sundianxing@hotmail.com
Research Domain of This Article
Virology
Article-Type of This Article
Original Article
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World J Gastroenterol. Dec 28, 2014; 20(48): 18284-18295 Published online Dec 28, 2014. doi: 10.3748/wjg.v20.i48.18284
Tricistronic hepatitis C virus subgenomic replicon expressing double transgenes
Xin Cheng, Xiang-Cui Gao, Jun-Ping Wang, Xin-Ying Yang, Yan Wang, Bao-Sheng Li, Fu-Biao Kang, Hai-Jun Li, Yue-Min Nan, Dian-Xing Sun
Xin Cheng, Xiang-Cui Gao, Jun-Ping Wang, Xin-Ying Yang, Yan Wang, Bao-Sheng Li, Fu-Biao Kang, Hai-Jun Li, Dian-Xing Sun, The Liver Disease Diagnosis and Treatment Center of PLA, Bethune International Peace Hospital, Shijiazhuang 050082, Hebei Province, China
Yue-Min Nan, Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang 050082, Hebei Province, China
Author contributions: Cheng X and Gao XC contributed equally to this work; Sun DX and Cheng X designed the research; Cheng X, Gao XC, Wang JP, Yang XY, Wang Y, Li BS, Kang FB and Li HJ performed the research; Sun DX, Cheng X, Gao XC and Nan YM analyzed the data; Sun DX and Cheng X wrote the paper.
Supported by Grants from the Study on Prevention and Control of Viral Hepatitis in the Key Program for Science and Technology Development of Hebei Province, No. 10276102D
Correspondence to: Dian-Xing Sun, MD, Professor of Medicine, The Liver Disease Diagnosis and Treatment Center of PLA, Bethune International Peace Hospital, 398 West Zhongshan Road, Shijiazhuang 050082, Hebei Province, China. sundianxing@hotmail.com
Telephone: +86-311-87978434 Fax: +86-311-87978434
Received: January 29, 2014 Revised: August 28, 2014 Accepted: October 15, 2014 Published online: December 28, 2014
Core Tip
Core tip: Two sequential RNA-binding motif protein 3 internal ribosome entry sites (IRESs) of 22 nucleotides were used to construct a tricistronic hepatitis C virus (HCV) replicon, initiating translation of humanized Renilla luciferase and HCV non-structural gene, along with HCV authentic IRES initiating translation of neomycin resistance gene. Intracellular HCV replicon RNA and expression of inserted foreign genes and HCV non-structural gene in cells transiently and stably transfected with tricistronic replicon RNA were comparable to those in cells transfected with traditional bicistronic HCV replicons. The novel replicon could be applied to evaluate replication inhibition efficacy of anti-HCV agents, except for interferon-α2b, which might be attributed to suppressed interferon response pathway.
