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World J Gastroenterol. Aug 28, 2014; 20(32): 11287-11296
Published online Aug 28, 2014. doi: 10.3748/wjg.v20.i32.11287
Published online Aug 28, 2014. doi: 10.3748/wjg.v20.i32.11287
Effect of GP73 silencing on proliferation and apoptosis in hepatocellular cancer
Yu-Long Zhang, You-Cheng Zhang, Wei Han, Yu-Min Li, Geng-Nian Wang, Shao Yuan, Feng-Xian Wei, Jia-Feng Wang, Jian-Jun Jiang, Ya-Wu Zhang, Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China
Yu-Long Zhang, You-Cheng Zhang, Wei Han, Yu-Min Li, Ya-Wu Zhang, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China
Yu-Long Zhang, You-Cheng Zhang, Wei Han, Yu-Min Li, Ya-Wu Zhang, Gansu Provincial-Level Key Laboratory of Digestive System Tumors, Lanzhou 730030, Gansu Province, China
Author contributions: Zhang YL, Zhang YC, Han W and Li YM designed the research; Zhang YL, Wang GN, Wei FX, Wang JF and Yuan S performed the research; Jiang JJ and Zhang YW contributed new reagents or analytic tools; Zhang YL and Zhang YC analyzed the data; Zhang YL and Zhang YC wrote the paper.
Supported by The Grant from Gansu Provincial-level Key Laboratory of Digestive System Tumors Open projects Research Fund, No. lzujbky-2011-t03
Correspondence to: You-Cheng Zhang, MD, PhD, Department of General Surgery, Lanzhou University Second Hospital, Cuiyingmen 82, Chengguan District, Lanzhou 730030, Gansu Province, China. zhangyouchengphd@163.com
Telephone: +86-931-8942287 Fax: +86-931-8942287
Received: December 25, 2013
Revised: February 28, 2014
Accepted: April 21, 2014
Published online: August 28, 2014
Revised: February 28, 2014
Accepted: April 21, 2014
Published online: August 28, 2014
Core Tip
Core tip: Stealth RNAi targeting Golgi protein (GP)73 gene sequence markedly reduced the expression of GP73 gene. Reduction of GP73 in Hep G2 cells and Bel7402 cells inhibited cell proliferation and induced apoptosis, however, terminal apoptosis occurred in Hep G2 cells, but early apoptosis occurred in Bel7402 cells. Reduced expression of GP73 gene might lead to a reduction in Bcl-2/ Bax ratio, an increase in cytochrome c, but a reduction in capase-3. GP73 might play an important role in proliferation and apoptosis in Hep G2 and Bel7402 cells.