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World J Gastroenterol. Aug 7, 2014; 20(29): 10062-10070
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10062
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10062
CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation
Hui-Hong Zhai, Juan Meng, Department of Digestive Diseases, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Jing-Bo Wang, Zhen-Xiong Liu, Yuan-Fei Li, State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710000, Shaanxi Province, China
Shan-Shan Feng, Surgery Laboratory, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Author contributions: Zhai HH conducted the laboratory studies, prepared the figures and tables and drafted the manuscript; Meng J performed siRNA transfection and cell culture; Wang JB conducted immunofluorescence; Liu ZX conducted Western blot; Li YF conducted cell cycle analysis; Feng SS constructed the siRNAs; all authors had read and approved the manuscript.
Supported by National Natural Science Foundation of China, No. 81072040
Correspondence to: Hui-Hong Zhai, MD, PhD, Department of Digestive Diseases, General Hospital of Ningxia Medical University, 804 Shengli Street, Xingqing Area, Yinchuan 750004, Ningxia Hui Autonomous Region, China. zhaihuihong@263.net
Telephone: +86-951-6744442 Fax: +86-951-4082981
Received: October 27, 2013
Revised: March 12, 2014
Accepted: April 30, 2014
Published online: August 7, 2014
Revised: March 12, 2014
Accepted: April 30, 2014
Published online: August 7, 2014
Core Tip
Core tip: Calcyclin binding protein, also called Siah-1 interacting protein (CacyBP/SIP), is a component of the ubiquitination pathway. It can translocate to the cell nucleus. In colon cancer cells, it was found that gastrin, epidermal growth factor, prostaglandin E2 and hypoxia can induce CacyBP/SIP nuclear translocation. Our previous study found that CacyBP/SIP-positive staining was observed in the cytoplasm and nuclei of some cancer cells in gastric cancer tissues. Thus, there is considerable interest in determining whether CacyBP/SIP can translocate into the nucleus or not in gastric cancer cells, and whether nuclear translocation of CacyBP/SIP mediates the proliferation of gastric cancer cells.