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World J Gastroenterol. Jun 7, 2014; 20(21): 6560-6572
Published online Jun 7, 2014. doi: 10.3748/wjg.v20.i21.6560
Published online Jun 7, 2014. doi: 10.3748/wjg.v20.i21.6560
Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation
Jennifer Raisch, Emmanuel Buc, Mathilde Bonnet, Pierre Sauvanet, Emilie Vazeille, Amélie de Vallée, Denis Pezet, Richard Bonnet, Marie-Agnès Bringer, Arlette Darfeuille-Michaud, Clermont Université, UMR1071 Inserm/Université d’Auvergne and INRA USC2018, 63000 Clermont-Ferrand, France
Emmanuel Buc, Pierre Sauvanet, Emilie Vazeille, Pierre Déchelotte, Claude Darcha, Denis Pezet, Richard Bonnet, Centre Hospitalier Universitaire, 63000 Clermont-Ferrand, France
Author contributions: Raisch J, Buc E, Bringer MA and Darfeuille-Michaud A conceived and designed the study, analysed data and drafted the manuscript; Bringer MA and Darfeuille-Michaud A contributed equally to the design and data analyses of this study; Raisch J, Buc E, Bonnet M, Vazeille E and Bringer MA performed experiments; Raisch J and Buc E contributed equally to this study; Buc E, Sauvanet P, de Vallée A, Pezet D and Bonnet R carried out the sample collection and the sample processing; Déchelotte P and Darcha C performed immunohistology analyses.
Supported by Ministère de l’Enseignement supérieur et de la Recherche, Inserm and Université d’Auvergne (UMR1071), INRA (USC-2018); and Grants from the Association F. Aupetit (AFA) and Ligue contre le cancer
Correspondence to: Marie-Agnès Bringer, PhD, Clermont Université, UMR1071 Inserm/Université d’Auvergne and INRA USC2018, 63000 Clermont-Ferrand, France. m-agnes.bringer@udamail.fr
Telephone: +33-4-7317 8371 Fax: +33-4-7317 8371
Received: October 25, 2013
Revised: February 10, 2014
Accepted: March 8, 2014
Published online: June 7, 2014
Revised: February 10, 2014
Accepted: March 8, 2014
Published online: June 7, 2014
Core Tip
Core tip: Tumors and mucosa of patients with colon cancer are abnormally colonized by Escherichia coli (E. coli) belonging to B2 phylogroup. The aim of the present study was to provide further insight into the characterization of colon cancer-associated E. coli. Despite their poor ability to adhere to and to invade intestinal epithelial cells in vitro, we showed that colon cancer-associated E. coli induce carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) expression, a receptor involved in adhesion of pathogenic E. coli. These bacteria were also able to persist and promote low grade inflammation and cell proliferation, in a chronic infection model of CEACAM6 expressing mice, highlighting their oncogenic potential.