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World J Gastroenterol. Aug 28, 2013; 19(32): 5340-5346
Published online Aug 28, 2013. doi: 10.3748/wjg.v19.i32.5340
Published online Aug 28, 2013. doi: 10.3748/wjg.v19.i32.5340
Clonality analysis of neuroendocrine cells in gastric adenocarcinoma
Ling-Ling Wang, Institute of Pathology and Forensic Medicine, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Ling-Ling Wang, Pathology Department, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China
Gen-You Yao, Xiao-Li Wei, Institute of Pathology and Forensic Medicine, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Zhong-Sheng Zhao, Zhejiang Provincial People’s Hospital, Hangzhou 310014, Zhejiang Province, China
Ru-Jun Xu, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China
Author contributions: Wang LL and Yao GY designed research; Wang LL performed research; Zhao ZS, Wei XL and Xu RJ contributed new reagents or analytic tools; Wang LL and Wei XL analyzed data; Wang LL wrote the paper.
Supported by The Natural Science Foundation of Zhejiang Province, China, No. Y2110133; the Zhejiang Provincial Medical Science Research Foundation, No. 2010KYA060
Correspondence to: Gen-You Yao, Professor of Medicine, Institute of Pathology and Forensic Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, China. yaogy@zju.edu.cn
Telephone: +86-571-87065701 Fax: +86-571-87914771
Received: March 24, 2013
Revised: June 13, 2013
Accepted: July 4, 2013
Published online: August 28, 2013
Revised: June 13, 2013
Accepted: July 4, 2013
Published online: August 28, 2013
Core Tip
Core tip: There have been only a few studies of neuroendocrine differentiation (NED) in gastric adenocarcinoma. Therefore, we studied the clonality of neuroendocrine (NE) cells in gastric adenocarcinoma using laser capture microdissection, microsatellite instability (MSI), loss of heterozygosity (LOH) and p53 mutation to evaluate the clonality of NED. NE and adenocarcinoma cells showed the same MSI, LOH or p53 mutation in most cases (27/30), they may originate from the same stem cells, but the remaining three cases showed different origins, which warrants further research.