Original Article
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World J Gastroenterol. Jun 28, 2013; 19(24): 3770-3780
Published online Jun 28, 2013. doi: 10.3748/wjg.v19.i24.3770
Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells
Jian Zou, Lin Mi, Xiao-Feng Yu, Jie Dong
Jian Zou, Lin Mi, Xiao-Feng Yu, Jie Dong, Department of Gastroenterology, Huadong Hospital, Fudan University, Shanghai 200040, China
Author contributions: Zou J and Yu XF contributed equally to this work; Zou J designed the research; Zou J, Mi L, Yu XF and Dong J performed the majority of the experiments; Zou J, Mi L and Dong J analysed the data; Zou J and Yu XF wrote the paper.
Supported by The Medical Guidance Projects of Shanghai Science Committee, No. 10411961800; National Natural Science Foundation of China, No. 81101617
Correspondence to: Xiao-Feng Yu, Professor, Department of Gastroenterology, Huadong Hospital, Fudan University, 221 Yan an Xi Road, Shanghai 200040, China. zoujian-dj@163.com
Telephone: +86-21-62483180 Fax: +86-21-62484981
Received: December 17, 2012
Revised: February 14, 2013
Accepted: March 21, 2013
Published online: June 28, 2013
Core Tip

Core tip: Of the 14-3-3 proteins, tumor-suppressor activity has most clearly been defined for 14-3-3σ. In the study, we constructed 14-3-3σ bait gene and expressed as a fusion to the GAL4 DNA-binding domain successfully. Using the yeast two-hybrid system, we found novel binding proteins from the HeLa cDNA library which closely interact with 14-3-3σ. Our results also suggest that 14-3-3σ may interact with potassium channel modulatory factor 1 (KCMF1) protein. The knock-down expression of 14-3-3σ and KCMF1 proteins significantly inhibited proliferation and colony formation of SW1116csc cells. So, 14-3-3σ and other proteins may be involved in proliferation and colony formation of human colon cancer stem cells.