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World J Gastroenterol. May 28, 2013; 19(20): 3143-3149
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3143
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3143
Value of circulating cell-free DNA in diagnosis of hepatocelluar carcinoma
Ken Chen, Hong Zhang, Li-Na Zhang, Dong-Feng Huang, Feng Li, Qun Wei, Jing Zhang, Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China
Shao-Qing Ju, Jing Qi, Center of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China
Author contributions: Chen K and Zhang H contributed equally to this work; Chen K and Zhang H designed the research; Chen K, Zhang H, Zhang LN, Ju SQ and Qi J performed the research; Ju SQ provided new reagents; Huang DF, Li F and Wei Q analyzed the data; Chen K, Zhang H and Zhang J wrote the manuscript.
Correspondence to: Hong Zhang, MD, Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Street, Nantong 226000, Jiangsu Province, China. zhangh111@sina.com
Telephone: +86-513-85052461 Fax: +86-513-85052588
Received: December 12, 2012
Revised: April 8, 2013
Accepted: April 18, 2013
Published online: May 28, 2013
Revised: April 8, 2013
Accepted: April 18, 2013
Published online: May 28, 2013
Core Tip
Core tip: Various techniques are available to quantify serum circulating free cell DNA (cfDNA), some of these techniques require abstraction and purification as there is only a very small amount of cfDNA in the serum. This study investigates the value of combined detection of cfDNA, α-fetal protein (AFP) and α L-fucosidase (AFU) for diagnosis of hepatocellular carcinoma. Quantitative analysis of cfDNA is sensitive and feasible, and the combined detection of cfDNA with AFP or AFU or both could improve the diagnostic sensitivity for hepatocellular carcinoma.