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World J Gastroenterol. May 28, 2013; 19(20): 3043-3051
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3043
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3043
MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults
Marisa C Alvarez, Juliana C Santos, Nathália Maniezzo, José Pedrazzoli Jr, Marcelo L Ribeiro, Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista 12916-900, SP, Brazil
Marisa C Alvarez, Marcelo L Ribeiro, Programa de Pós Graduação em Genética e Biologia Molecular, State University of Campinas, Campinas 13083-970, SP, Brazil
Marcelo S Ladeira, Departamento de Clínica Médica, UNESP, Botucatu 18618-970, SP, Brazil
Artur LC da Silva, Departamento de Genética, Universidade Federal do Pará, Belém 68400-000, PA, Brazil
Isabel CA Scaletsky, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo 04021-001, SP, Brazil
Author contributions: Ribeiro ML contributed to research design; Alvarez MC performed the research; Santos JC and Maniezzo N contributed to contributions of new reagents/analytical tools; Alvarez MC and Ribeiro ML contributed to data analysis; Alvarez MC wrote the manuscript; da Silva ALC performed the MSI analysis and sequencing; Ladeira MS, Scaletsky ICA and Pedrazzoli Jr J provided specimens.
Supported by The Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, No. 2009/01813-0 and 2008/02678-6; the Conselho Nacional de Desenvolvimento Científico e Tecnológico, No. 471088/2007-2
Correspondence to: Marcelo L Ribeiro, PhD, Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista 12916-900, SP, Brazil. marcelo.ribeiro@usf.edu.br
Telephone: +55-11-40348135 Fax: +55-11-40341825
Received: November 21, 2012
Revised: March 26, 2013
Accepted: April 9, 2013
Published online: May 28, 2013
Revised: March 26, 2013
Accepted: April 9, 2013
Published online: May 28, 2013
Core Tip
Core tip: Gastric carcinogenesis is a multistep process that is triggered by Helicobacter pylori (H. pylori) infection and characterised by multiple genetic and epigenetic alterations, including the DNA repair genes. To date, few advances have been made to determine the time duration required for to H. pylori infection to induce such epigenetics alteration and thus potentially induce gastric carcinogenesis. The results presented in this study indicate that the methylation of the MGMT and MLH1 promoter regions might depend on the duration of infection because these methylation events were not observed in children.