Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 21, 2019; 25(15): 1828-1839
Published online Apr 21, 2019. doi: 10.3748/wjg.v25.i15.1828
Lysyl oxidase and hypoxia-inducible factor 1α: biomarkers of gastric cancer
Ya-Lin Han, Li Chen, Rui Qin, Guan-Qing Wang, Xiao-Hua Lin, Guang-Hai Dai
Ya-Lin Han, Li Chen, Rui Qin, Guan-Qing Wang, Guang-Hai Dai, Department of Medical Oncology, Chinese PLA General Hospital, Beijing 100853, China
Xiao-Hua Lin, Department of Oncology, the General Hospital of PLA Rocket Force, Beijing 100088, China
Author contributions: Dai GH and Chen L designed and provided ideas for the project; Han YL wrote and edited the manuscript; Han YL, Chen L and Qin R performed immunohistochemical staining; Han YL analyzed all the data; Han YL and Lin XH conducted RNA separation, cDNA synthesis and qRT-PCR analysis; Han YL, Qin R and Wang GQ collected clinical samples from our hospital.
Supported by the grants from the Military Medical Science and Technology Youth Training Program Project (16QNP146).
Institutional review board statement: This study was conducted with permission from the Institutional Research Ethics Committee of Chinese PLA General Hospital.
Institutional animal care and use committee statement: This study did not include the animal experiments.
Conflict-of-interest statement: The authors declare no potential conflicts of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: This study did not include the animal experiments.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Guang-Hai Dai, PhD, Professor, Department of Medical Oncology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China. handai713@126.com
Telephone: +86-10-66937231
Received: January 19, 2019
Peer-review started: January 21, 2019
First decision: February13, 2019
Revised: February 21, 2019
Accepted: March 1, 2019
Article in press: March 2, 2019
Published online: April 21, 2019
ARTICLE HIGHLIGHTS
Research background

To study whether lysyl oxidase (LOX) and hypoxia-inducible factor 1α (HIF1α) can be used as prognostic and predictive biomarkers in gastric cancer (GC).

Research motivation

To provide the prognostic and predictive biomarkers for treating GC.

Research objectives

To explore the interaction between cancer and factors in the metabolic microenvironment and determine predictive biomarkers of GC.

Research methods

Patients and samples: This work is difficult and requires patient approval.

RNA extraction and cDNA synthesis: This work requires a lot of time, and so the manipulator should be patient. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR): The manipulator should add the samples accurately during qRT-PCR analysis. Immunohistochemistry: The experiments take a long time, and the manipulator should perform the experiments step by step. Statistical analysis: All of the experiments were repeated at least three times, and the statistical analysis was then performed.

Research results

The expression levels of LOX and HIF1α increased in the tumor tissues and blood samples of patients with GC. The expression levels of LOX and HIF1α were related to the clinicopathological characteristics and prognosis of GC.

Research conclusions

LOX and HIF1α can be used as prognostic and predictive biomarkers for the treatment of GC. Our study indicated that the expression of LOX and HIF1α was upregulated in patients with GC compared with the control group. In addition, the expression of LOX and HIF1α was related to the clinicopathological characteristics and prognosis of GC.

Research perspectives

The present study suggested that LOX and HIF1α might be used as both prognostic and predictive biomarkers for GC, and provided a link between LOX, HIF1α and GC.