Colorectal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2003; 9(9): 1995-1998
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.1995
Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell
Xue-Liang Guo, Geng-Jin Lin, Hong Zhao, Yong Gao, Li-Ping Qian, San-Rong Xu, Li-Na Fu, Qing Xu, Jie-Jun Wang
Xue-Liang Guo, Geng-Jin Lin, Hong Zhao, Li-Ping Qian, San-Rong Xu, Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200032, China
Yong Gao, Qing Xu, Jie-Jun Wang, Medical oncology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
Li-Na Fu, Department of Gastroenterology, Shandong Provincial Hospital, Ji’nan 250021, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Geng-Jin Lin, Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200032, China.
Telephone: +86-21-65790000-4036
Received: November 12, 2002
Revised: December 15, 2002
Accepted: December 30, 2002
Published online: September 15, 2003

AIM: To study the effects of non-cytotoxic concentrations of docetaxel on some important angiogenic factors of LS174T Cells.

METHODS: The non-cytotoxic concentration of docetaxel and the activity of gelatinase were determined with MTT and gelatin zymography respectively, the expression of VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor), MMP (matrix metalloproteinase) 2 and MMP 9 was investigated with RT-PCR and Western blot.

RESULTS: The maximum non-cytotoxic concentration of docetaxel on LS174T Cells was 1.0 ng/mL. Compared with the solvent control group, 0.1, 0.5, 1.0 ng/mL of docetaxel could downregulate the expression of VEGF, bFGF, MMP 2 and MMP 9 and suppress the activity of gelatinase.

CONCLUSION: Our study suggests that the non-cytotoxic concentrations of docetaxel have strong antiangiogenic activity on LS174T Cells, which suggests docetaxel may be a promising antiangiogenic agent.

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