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Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2003; 9(8): 1863-1865
Published online Aug 15, 2003. doi: 10.3748/wjg.v9.i8.1863
Relationship between nuclear morphometry, DNA content and resectability of pancreatic cancer
Yin-Cheng He, Wei Peng, Jian-Guo Qiao, Jun Cao, Ji-Wei Chen
Yin-Cheng He, Jian-Guo Qiao, Jun Cao, Ji-Wei Chen, Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
Wei Peng, Medical College, Jingmen Technical College, Jingmen 448000, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Yin-Cheng He, Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China.
Telephone: +86-27-67812963
Received: May 11, 2003
Revised: May 12, 2003
Accepted: June 4, 2003
Published online: August 15, 2003

AIM: To investigate the association of nuclear morphometry and DNA content with resectability of pancreatic cancer.

METHODS: A total of 36 patients with pancreatic adenocarcinoma were divided into resectable group and unresectable group. The nuclear morphometry and DNA contents of tumor cells were analyzed by IBAS autoimagine analyzer from paraffin-embedded materials. Localization size, histological type and grade, and clinical stage of the tumor were evaluated. Factors influencing resectability of pancreatic cancer were investigated using stepwise regression analysis.

RESULTS: Statistical significance was found in nuclear DNA content (integrated optical density, IOD) of tumor cells (1.64 ± 0.41 vs 2.96 ± 0.55), DNA ploidy, ages (46.5 ± 5.3 years vs 58.6 ± 0.7 years) and tumor volumes (298.1 ± 101.5 cm3vs 634.7 ± 512.5 cm3) in both groups (P < 0.05), and no difference was found in the nuclear morphometry (P > 0.05). The rates of diploid/tetraploid and aneuploid were 66.7% and 33.3% in resectable group respectively, and 38.9% and 62.1% in unresectable group, respectively (P < 0.05). IOD (X12), ploidy status (X13) and clinical stage (X3) were radical resectable indicators with statistical significance. The regression equation for resectability was Y = -9.2053 + 3.5428X12 + 2.5390X13 - 2.3001X3 (RR = 0.8780, P < 0.01).

CONCLUSION: There is a high correlation between resectability of pancreatic cancers and their DNA contents, DNA ploidy status and clinical stage.

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