Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1427
Revised: June 4, 2002
Accepted: June 12, 2002
Published online: July 15, 2003
AIM: To study the preventive effect of hydrotalcite on gastric mucosal injury in rat induced by taurocholate, and to investigate the relationship between the protective mechanism of hydrotalcite and the expression of trefoil factor family 2 (TFF2) mRNA and c-fos protein.
METHODS: Forty five male Wistar rats were randomly divided into hydrotalcite group, ranitidine group and control group. Gastric mucosal injury was induced by introgastric acidified taurocholate. OD value of TFF2 mRNA expression in gastric mucous cells was determined by hybridization and computer image analysis system. OD value of c-fos protein expression in gastric mucous cells was measured by immunohistochemistry and computer image analysis system.
RESULTS: The gross mucosal injury index in hydrotalcite group was significantly lower than that in ranitidine group and control group (8.60 ± 2.20 vs 16.32 ± 4.27, 29.53 ± 5.39; P < 0.05, P < 0.01). The expression level of TFF2 mRNA in hydrotalcite group was markedly higher than that in ranitidine group and control group (0.56 ± 0.09 vs 0.30 ± 0.05, 0.28 ± 0.03, P < 0.05). The OD value of c-fos protein in hydrotalcite group was higher than that in ranitidine group and control group (0.52 ± 0.07 vs 0.31 ± 0.04, 0.32 ± 0.05, P < 0.05).
CONCLUSION: Hydrotalcite can protect gastric mucosal injury in rats induced by taurocholate, which may be related to the increased expression of TFF2 and c-fos protein.