Wu XT, Li JS, Zhao XF, Li N, Ma YK, Zhuang W, Zhou Y, Yang G. Effects of n-3 fatty acid, fructose-1, 6-diphosphate and glutamine on mucosal cell proliferation and apoptosis of small bowel graft after transplantation in rats. World J Gastroenterol 2003; 9(6): 1323-1326 [PMID: 12800249 DOI: 10.3748/wjg.v9.i6.1323]
Corresponding Author of This Article
Professor Xiao-Ting Wu, Department of General Surgery, West China Hospital, Sichuan University, 37 Guo Xue Rd., Chengdu 610041, Sichuan Province, China. wxt1@medmail.com.cn
Article-Type of This Article
Basic Research
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World J Gastroenterol. Jun 15, 2003; 9(6): 1323-1326 Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1323
Effects of n-3 fatty acid, fructose-1, 6-diphosphate and glutamine on mucosal cell proliferation and apoptosis of small bowel graft after transplantation in rats
Xiao-Ting Wu, Jie-Shou Li, Xiao-Fei Zhao, Ning Li, Yu-Kui Ma, Wen Zhuang, Yong Zhou, Gang Yang
Xiao-Ting Wu, Yu-Kui Ma, Wen Zhuang, Yong Zhou, Gang Yang, Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Jie-Shou Li, Ning Li, Research Institute of General Surgery, Nanjing General Hospital of PLA, Clinical School of Medical College, Nanjing University, Nanjing 210002, Jiangsu Province, China
Xiao-Fei Zhao, Sichuan Reproductive Health Institute, Chengdu 610041, Sichuan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the State Education Commission Research Foundation for Returned Overseas Chinese Scholars Abroad (1997) 436
Correspondence to: Professor Xiao-Ting Wu, Department of General Surgery, West China Hospital, Sichuan University, 37 Guo Xue Rd., Chengdu 610041, Sichuan Province, China. wxt1@medmail.com.cn
Telephone: +86-28-85422480 Fax: +86-28-85422411
Received: September 13, 2002 Revised: November 4, 2002 Accepted: November 21, 2002 Published online: June 15, 2003
Abstract
AIM: To evaluate the effects of n-3 fatty acids (n-3FA), fructose-1, 6-diphosphate (FDP) and glutamine (GLN) on mucosal cell proliferation and apoptosis of small bowel graft.
METHODS: One hundred and ninety-six inbred strain Wistar rats were grouped as donors and recipients, and underwent heterotopic small bowel transplantation (SBT). n-3FA, FDP and GLN were administered via gastric tube as well as venous infusion for 10 d before and after surgery, respectively. The proliferation and apoptosis of mucosal cells were analyzed with flow cytometry and in situ cell death detection kits.
RESULTS: Apparent apoptosis and minor proliferation of mucosal cells of small bowel graft after transplantation were observed. A higher mucosal cell proliferative index and lower apoptotic index were found in all small bowel grafts after supplying with n-3FA, FDP and GLN.
CONCLUSION: Nutritional support with n-3FA, FDP and GLN promotes mucosal cell proliferation significantly, and prevents mucosal cell from undergoing apoptosis with different degrees. These regulatory effects on the apoptosis alter the structure and absorption function of transplanted small bowel favorably.
