Esophageal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2003; 9(10): 2143-2148
Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2143
Separation and identification of differentially expressed nuclear matrix proteins between human esophageal immortalized and carcinomatous cell lines
Xing-Dong Xiong, En-Min Li, Li-Yan Xu, Hai-Bin Chen, Ling Chen, Wei-Jia Cai, Ya-Li Han, Zhong-Ying Shen, Yi Zeng
Xing-Dong Xiong, En-Min Li, Department of Biochemistry and Molecular Biology, Medical College, Shantou University, Shantou 515031, Guangdong Province, China
Xing-Dong Xiong, En-Min Li, Li-Yan Xu, Zhong-Ying Shen, Wei-Jia Cai, Institute of Oncologic Pathology, Medical College, Shantou University, Shantou 515031, Guangdong Province, China
Hai-Bin Chen, Department of Histology and Embryology, Medical College, Shantou University, Shantou 515031, Guangdong Province, China
Ling Chen, Molecular Biological Center, Medical College, Shantou University, Shantou 515031, Guangdong Province, China
Ya-Li Han, Department of Biology, Shantou University, Shantou 515031, Guangdong Province, China
Yi Zeng, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39900069, No.30170428; Natural Science Foundation of Guangdong Province, No.990799, No.010431; College Natural Science Foundation of Guangdong province, No.200033; Medical Scientific Foundation of Guangdong Province, No.A2001419 and Research and Development Foundation of Shantou University, No.L0004, No.L00012
Correspondence to: Dr. En-Min Li, Department of Biochemistry and Molecular Biology, Medical College, Shantou University, 22 Xinling Road, Shantou 515031, Guangdong Province, China. nmli@stu.edu.cn
Telephone: +86-754-8532720 +86-754-8900847
Received: November 19, 2002
Revised: March 4, 2003
Accepted: March 11, 2003
Published online: October 15, 2003
Abstract

AIM: To separate and identify differentially expressed nuclear matrix proteins (NMPs) between the immortalized human esophageal epithelial cell line (SHEE) and the malignantly transformed esophageal carcinoma cell line (SHEEC), and to provide new ways for finding specific markers and the pathogenesis of esophageal carcinoma.

METHODS: SHEE and SHEEC cell lines were used to extract NMPs. The quality of NMPs was monitored by Western blot analysis including DNA topoisomerase IIα, proliferation cell nuclear antigen (PCNA) and histone. NMPs of SHEE and SHEEC were analyzed by two-dimensional electrophoresis (2-DE), silver staining and PDQuest6.2 image analysis software. Three spots in which the differentially expressed NMPs were more obvious, were selected and analyzed with matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS) and database search.

RESULTS: Western blot analysis revealed that DNA topoisomerase IIα and PCNA were detected, and the majority of histones were deleted in NMPs of SHEE and SHEEC. After 2-DE image analysis by PDQuest6.2 software, the 2-DE maps were detected with an average of 106 ± 7.1 spots in SHEE and 132 ± 5.0 spots in SHEEC. Most of them were matched one another (r = 0.72), only 16 protein spots were found differing in intensity. Three NMPs including cytoskeletal tropomyosin, FK506-binding protein 6, similar to retinoblastoma binding protein 8 were preliminarily identified by MALDI- TOF-MS.

CONCLUSION: These differentially expressed NMPs may play an important role during malignant transformation from SHEE to SHEEC. Their separation and identification will contribute to searching for specific markers and probing into the pathogenesis of esophageal carcinoma.

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