Basic Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 15, 2003; 9(1): 148-151
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.148
Cloning and analysizing the up-regulated expression of transthyretin-related gene (LR1) in rat liver regeneration following short interval successive partial hepatectomy
Cun-Shuan Xu, Yu-Chang Li, Jun-Tang Lin, Hui-Yong Zhang, Yun-Han Zhang
Cun-Shuan Xu, Yu-Chang Li, Jun-Tang Lin, Hui-Yong Zhang, College of Life Science, Henan Normal University, Xinxiang 453002, Henan Province, China
Yun-Han Zhang, Henan Key Laboratory for Tumor Pathology, Zhengzhou 450052, Henan Province, China
Author contributions: All authors contributed equally to the work.
Supported by grants from National Natural Science Foundation of China, No. 39970362; Tackle Key of Scientific and Technical Problem of Henan Province, No. 0122031900
Correspondence to: Prof. Dr. Cun-Shuan Xu, College of Life Science, Henan Normal University, Xinxiang, 453002, Henan Province, China. xucs@x263.net
Telephone: +86-373-3326341/3326609 Fax: +86-373-3326524
Received: March 12, 2002
Revised: April 16, 2002
Accepted: April 20, 2002
Published online: January 15, 2003
Abstract

AIM: Cloning and analysizing the up-regulated expression of transthyretin-related gene following short interval successive partial hepatectomy (SISPH) to elucidate the mechanism of differentiation, division, dedifferentiation and redifferentiation in rat liver regeneration (LR).

METHODS: Lobus external sinister and lobus centralis sinister, lobus centralis, lobus dexter, lobus candatus were removed one by one from rat liver at four different time points 4, 36, 36 and 36 hr (total time: 4 hr, 40 hr, 76 hr, 112 hr) respectively. Suppression subtractive hybridization (SSH) was carried out by using normal rat liver tissue as driver and the tissue following short interval successive partial hepatectomy (SISPH) as tester to construct a highly efficient forward-subtractive cDNA library. After screening, an interested EST fragment was selected by SSH and primers were designed according to the sequence of the EST to clone the full-length cDNA fragment using RACE (rapid amplification of cDNA end). Homologous detection was performed between the full-lenth cDNA and Genbank.

RESULTS: Forward suppression subtractive hybridization (FSSH) library between 0 h and 112 h following SISPH was constructed and an up-regulated full-length cDNA (named LR1), which was related with the transthyretin gene, was cloned by rapid amplification of cDNA end. It was suggested that the gene is involved in the cellular dedifferentiation in LR following SISPH.

CONCLUSION: Some genes were up-regulated in 112 h following SISPH in rat. LR1 is one of these up-regulated expression genes which may play an important role in rat LR.

Keywords: $[Keywords]