Liver Cancer
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2002; 8(4): 631-637
Published online Aug 15, 2002. doi: 10.3748/wjg.v8.i4.631
Intergrin gene expression profiles of humanhepatocellular carcinoma
Lian-Xin Liu, Hong-Chi Jiang, Zhi-Hua Liu, Jing Zhou, Wei-Hui Zhang, An-Long Zhu, Xiu-Qin Wang, Min Wu
Lian-Xin Liu, Hong-Chi Jiang, Wei-Hui Zhang, An-Long Zhu, Department of Surgery, the First Clinical College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Lian-Xin Liu, Zhi-Hua Liu, Jing Zhou, Xiu-Qin Wang, Min Wu, National Laboratory of Molecular Oncology, Department of Cell Biology, Cancer Institute, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100021, China
Author contributions: All authors contributed equally to the work.
Supported by China Key Program on Basic Research, Grant Number: Z19-01-01-02; Chinese Climbing Project No.18; Youth Natural Scientific Foundation of Heilongjiang Province
Correspondence to: Professor Zhi-Hua Liu, National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Science & Peking Union Medical College, Panjiayuan, Chaoyang District, Beijing 100021, China.
Telephone: +86-451-3668999 Fax: +86-451-3670428
Received: May 3, 2002
Revised: June 12, 2002
Accepted: June 15, 2002
Published online: August 15, 2002

AIM: To investigate gene expression profiles of intergrin genes in hepatocellular carcinoma (HCC) through the usage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Northern blot.

METHODS: Hybridization of cDNA array membrane was performed with α32P-labeled cDNA probes synthesized from RNA isolated from hepatocellular carcinoma and adjacent non-cirrhotic liver. AtlasImage, which is a software specific to array, was used to analyze the result. RT-PCR of 24 pairs specimen and Northern blot of 4 pairs specimen were used to confirm the expression pattern of some intergrin genes identified by Atlas arrays hybridization.

RESULTS: Among 588 genes spotted in membrane, 17 genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, beta1, beta7 and beta8 in HCC. Whereas there were no genes down-regulated in HCC. RT-PCR and Northern blot analysis of intergrin beta1 gene gave results consistent with cDNA array findings.

CONCLUSION: Investigation of these intergrin genes should help to disclose the molecular mechanism of the cell adhesion, invasive and metastasis of HCC. A few genes are reported to have changed in HCC for the first time. The quick and high-throughout method of profiling gene expression by cDNA array provides us overview of key factors that may involved in HCC, and may find the clue of the study of HCC metastasis and molecular targets of anti-metastasis therapy. The precise relationship between the altered genes and HCC is a matter of further investigation.

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