Gastric Cancer
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2002; 8(4): 613-618
Published online Aug 15, 2002. doi: 10.3748/wjg.v8.i4.613
Expression of focal adhesion kinase and α5 and β1 integrins in carcinomas and its clinical significance
Jian-Min Su, Lu Gui, Yi-Ping Zhou, Xi-Liang Zha
Jian-Min Su, Yi-Ping Zhou, Department of Chemistry, FuDan University, Shanghai 200032, China
Lu Gui, Department of Pathology, Jinshan Hospital, FuDan University Medical Center, Shanghai 200032, China
Xi-Liang Zha, Department of Biochemistry, FuDan University Medical Center, Shanghai 200032, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China, No.39970373 and the grant from the Science Committee of Shanghai, No.00JC14042
Correspondence to: Xi-Liang Zha, Department of Biochemistry, FuDan University Medical Center, 138 Yixueyuan Road, Shanghai 200032, China. xlzha@shmu.
Telephone: +86-21-64041900-2312 or 2696
Received: January 11, 2002
Revised: February 4, 2002
Accepted: February 9, 2002
Published online: August 15, 2002

AIM: To detect the expression pattern of FAK (focal adhesion kinase) and integrin α5 and β1 subunits in different kinds of cancerous tissues and to study their correlation with clinicopathological data including tumor type, grade and lymph node status.

METHODS: Using an immunohistochemical technique, we examined the expression of FAK and integrin and 1 subunits in cancerous and noncancerous tissues obtained from 75 patients with gastric carcinomas, 21 colorectal carcinomas, 16 hepatocellular carcinomas, 20 uterocervical carcinomas, and 20 breast carcinomas.

RESULTS: The staining of FAK was stronger in cancerous than in noncancerous areas. Enhanced expression of FAKwas detected in poor-differentiated carcinoma of the stomach and colorectum. Tumors with lymph node metastases had more FAK protein than those without metastases. In addition, the deeper the extent of tumor infiltration, the higher the FAK expression. The expression of integrin α5 and β1 subunits was lower in cancerous areas than in noncancerous areas, but it was higher in well-differentiated cancerous tissues than in poor differentiated tissues. The relationship between the expression of integrin α5 and β1 subunits and infiltration or metastasis was not significant. Cancerous tissues with stronger FAK expression (++ or +++) also had a higher expression of integrin α5 and β1 subunits in the tumor and its unaffected margins.

CONCLUSION: FAK is a better marker for carcinogenesis and the progression of cancer than integrin α5 or β1 subunit, and it may be not only a transformation-linked enzyme but also a progression-linked enzyme.

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