Li Y, Yang L, Cui JT, Li WM, Guo RF, Lu YY. Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells. World J Gastroenterol 2002; 8(2): 208-212 [PMID: 11925593 DOI: 10.3748/wjg.v8.i2.208]
Corresponding Author of This Article
Dr. You-Yong Lu, Beijing Institute for Cancer Research, Beijing Laboratory of Molecular Oncology, School of Oncology, Peking University, 1 Da-Hong-Luo-Chang Street, Western District, Beijing 100034, China.yongylu@public.bta.net.cn
Article-Type of This Article
Gastric Cancer
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World J Gastroenterol. Apr 15, 2002; 8(2): 208-212 Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.208
Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells
Yong Li, Lin Yang, Jian-Tao Cui, Wen-Mei Li, Rui-Fang Guo, You-Yong Lu
Yong Li, Jian-Tao Cui, Wen-Mei Li, You-Yong Lu, Beijing Institute for Cancer Research, Beijing Laboratory of Molecular Oncology, School of Oncology, Peking University, Beijing 100034, China
Lin Yang, Department of Gastroenterology, PLA Institute for Digestive Diseases, First Affiliated Hospital of First Military Medical University, Chinese, Guangzhou, 510000, Guangdong Province, China
Rui-Fang Guo, Department of Physiology, Neimenggu Medical College, Huhehaote, 010000, Neimengguzizhiqu, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Scientific Foundation of China (NSFC3962526) and National High-Technology Project-863 (102-10-01-04).
Correspondence to: Dr. You-Yong Lu, Beijing Institute for Cancer Research, Beijing Laboratory of Molecular Oncology, School of Oncology, Peking University, 1 Da-Hong-Luo-Chang Street, Western District, Beijing 100034, China.yongylu@public.bta.net.cn
Telephone: +86-10-66163061 Fax: +86-10-66175832
Received: August 9, 2001 Revised: November 1, 2001 Accepted: November 14, 2001 Published online: April 15, 2002
Abstract
AIM: To elucidate molecular mechanism of chemopreventive efficacies of garlic against human gastric cancer (HGC).
METHODS: HGC cell line BGC823 was treated with Allitridi (a kind of garlic extract) and Allitridi-treated and parental BGC823 cDNA libraries were constructed respectively by using λZAP II vector. cDNA Representational Difference Analysis (cDNA RDA) was performed using BamH I cutting-site and abundant cDNA messages provided by the libraries. Northern blot analysis was applied to identify the obtained difference products.
RESULTS: Two specific cDNA fragments were obtained and characterized to be derived from homo sapiens folate receptorα (FRα) gene and calcyclin gene respectively. Northern blot results showed a 4-fold increase in FRα gene expression level and 9-fold increase in calcyclin mRNA level in BGC823 cells after Allitridi treatment for 72 h.
CONCLUSION: The method of cDNA RDA based on cDNA libraries combines the high specificity of cDNA RDA with abundant cDNA messages in cDNA library; this expands the application of cDNA library and increases the specificity of cDNA RDA. Up-regulation of FRα gene and calcyclin gene expressions induced by Allitridi provide valuable molecular evidence for the efficacy garlic in treating HGC as well as other diseases.
