Published online Feb 15, 2002. doi: 10.3748/wjg.v8.i1.79
Revised: August 20, 2001
Accepted: August 23, 2001
Published online: February 15, 2002
AIM: To study the anti-tumor effect of resveratrol alone and the synergistic effects of resveratrol with 5-FU on the growth of H22 cells line in vitro
METHODS: The number of cells was measured by MTT method,the morphological changes of H22 cells were investigated under microscopy and electron microscopyq.
RESULTS: Resveratrol inhibited the growth of hepatoma cells line H22 in a dose- and time-dependent manner. IC50 of the resveratrol on H22 cells was 6.57 mg·L-1. The synergistic anti-tumor effects of resveratrol with 5-FU increased to a greater extent than for H22 cells treated with 5-FU alone (70.2% vs 28.4%)(P < 0.05). Under microscope and electron microscope, characteristics of apoptosis such as typical apoptotic bodies were commonly found in tumor cells in the drug-treated groups.
CONCLUSION: Resveratrol can suppresses the growth of H22 cells in vitro, its anti-tumor activity may occur through the induction of apoptosis.