Original Research
Copyright ©The Author(s) 2001. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2001; 7(4): 537-541
Published online Aug 15, 2001. doi: 10.3748/wjg.v7.i4.537
Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection
Fu-Sheng Wang, Li-He Xing, Ming-Xu Liu, Chuan-Lin Zhu, Hui-Gang Liu, Hui-Fen Wang, Zhou-Yun Lei
Fu-Sheng Wang, Li-He Xing, Ming-Xu Liu, Hui-Gang Liu, Zhou-Yun Lei, Division of Biological Engineering, Beijing Institute of Infectious Diseases, Beijing Hospital of Infectious Diseases, Beijing 100039, China
Chuan-Lin Zhu, Hui-Fen Wang, Fourth Department of Liver Diseases, Beijing Institute of Infectious Diseases, Beijing Hospital of Infectious Diseases, Beijing 100039, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China, No.39970831.
Correspondence to: Dr. Fu-Sheng Wang, Division of Biological Engineering, Beijing Institute of Infectious Diseases, 26 Fengtai Road, Beijing 100039, China. fswang@public.bta.net.cn
Telephone: 0086-10-66933332 Fax: 0086-10-63831870
Received: February 28, 2001
Revised: March 14, 2001
Accepted: March 21, 2001
Published online: August 15, 2001

AIM: To identify the property of dendritic cells (DCs) of peripheral blood monocytes (PBMC) in patients with chronic HBV infection.

METHODS: Twenty patients with persistent HBV infection were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GM-CSF, IL-4, FLt-3, TNF-α and 100 mL·L-1 of fetal calf serum for a total of 10-12 d. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation, mytomycin C-treated DC were incubated with allogenic T cells.

RESULTS: A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLA-DR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0.84 for CD86, P < 0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCs simultaneously increased compared with healthy subjects (86 ± 15 vs 170 ± 22 μmol·L-1, P < 0.05).

CONCLUSION: The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV.

Keywords: dendritic cells/pathology, hepatitis B, chronic/pathology, nitric oxide/analysis, interleukin-10/analysis, interleuki-12/analysis