Published online Oct 15, 2000. doi: 10.3748/wjg.v6.i5.709
Revised: May 20, 2000
Accepted: June 2, 2000
Published online: October 15, 2000
AIM: To clone expressed genes associated with repair of irradiation-damaged mice intestinal gland cells treated by small intestinal RNA, and to explore the molecular mechanism of exogenous nucleic acids improving repair of intestinal crypt.
METHODS: The animal mode of test group and control group was established, forty-five mice being irradiated by γ ray were treated with small intestinal RNA as test group, forty mice being irradiated by γ ray were treated with physiological saline as control group, five mice without irradiation were used as normal control, their jejunal specimens were collected respectively at 6 h, 12 h, 24 h, 4 d and 8 d after irradiation. Then by using LD-PCR based on subtractive hybridization, these gene fragments differentially expressed between test group and control group were obtained, and then were cloned into T vectors as well as being sequenced. Obtained sequences were screened against. GeneBank, if being new sequences, they were submitted to GeneBank.
RESULTS: Ninety clones were associated with repair of irradiation-damaged intestinal gland cells treated by intestinal RNA. These clones from test group of 6 h, 12 h, 24 h, 4 d and 8 d were respectively 18, 22, 25, 13, 12. By screening against GeneBank, 18 of which were new sequences, the others were dramatically similar to the known sequences, mainly similar to hsp, Nmi, Dutt1, alkaline phosphatase, homeobox, anti-CEA ScFv antibody, arginine/serine kinase and BMP-4, repA. Eighteen gene fragments were new sequences, their accept numbers in GeneBank were respectively AF240164-AF240181.
CONCLUSION: Ninety clones were obtained to be associated with repair of irradiation-damaged mice intestinal gland cells treated by small intestinal RNA, which may be related to abnormal expression of genes and matched proteins of hsp, Nmi, Dutt1, Na, K-ATPase, alkalineph-osphatase, glkA, single stranded replicative centromeric gene as well as 18 new sequences.