Zhang SZ, Liang JJ, Qi ZT, Hu YP. Cloning of the non-structural gene 3 of hepatitis C virus and its inducible expression in cultured cells. World J Gastroenterol 1999; 5(2): 125-127 [PMID: 11819409 DOI: 10.3748/wjg.v5.i2.125]
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World J Gastroenterol. Apr 15, 1999; 5(2): 125-127 Published online Apr 15, 1999. doi: 10.3748/wjg.v5.i2.125
Cloning of the non-structural gene 3 of hepatitis C virus and its inducible expression in cultured cells
Shu-Zhong Zhang, Jia-Jing Liang, Zhong-Tian Qi, Yi-Ping Hu
Shu-Zhong Zhang, Jia-Jing Liang, Yi-Ping Hu, Department of Cell Biology, Department of Basic Medicine, Second Military Medical University, Shanghai 200433, China
Zhong-Tian Qi, Department of Microbiology, Department of Basic Medicine, Second Military Medical University, Shanghai 200433, China
Shu-Zhong Zhang, male, born on December 18, 1968 in Jinan, Shandong, China, graduated from Second Military Medical University in July 1997, lecturer, engaged in the researches of transgenic animals under the instruction of Professor HU Yi-Ping, having three papers published.
Author contributions: All authors contributed equally to the work.
Correpondence to: Yi-Ping Hu, Department of Cell Biology, Department of Basic Medicine, Second Military Medical University, Shanghai 200433, China
Telephone: +86-21-25070240
Received: November 9, 1998 Revised: January 16, 1999 Accepted: January 26, 1999 Published online: April 15, 1999
Abstract
AIM To study the inducible expression of hepatitis C virus ns3 gene (HCV-ns3) in eukaryotic cells.
METHODS The ns3 gene was obtained from plasmid pBns3 by polymerase chain reaction and inserted into the cloning vector pGEM-T. Then, the ns3 was subcloned into the vector pMSG to generate dexamethasone (DM)inducible expression plasmid pMSG-ns3. CHO cells were transfected by pMSG-ns3 using calcium phosphate precipitation method and cultivated for 12 h-24 h. The transfected cells were induced with DM and the transient expression of NS3 protein was analyzed by ELISA and Western-blot methods.
RESULTS After treated with 3 × 10-8 mol/L DM, the expression of NS3 was observed in the transfected CHO cells. A slightly higher level of NS3 was shown along with the time of DM treatment.
CONCLUSION The inducible expressing vector pMSG-ns3 might be helpful for further studies of the characteristics of the ns3 gene in vivo.
