Cao GD, Wang SW, Wu SS, Li HF, Zhang WG. Retrovirus-ediated antisense RNA to bcl-2 alter the biological behavior of stomach carcinoma MGC-03 cell lines. World J Gastroenterol 1998; 4(Suppl2): 41-44 [DOI: 10.3748/wjg.v4.iSuppl2.41]
Corresponding Author of This Article
Dr. Guo-Dong Cao, Central Laboratory, People’s Hospital, Beijing Medical University, Beijing 100044, China
Article-Type of This Article
Original Articles
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Guo-Dong Cao, Sheng-Wu Wang, Sha-Sha Wu, Central Laboratory, People’s Hospital, Beijing Medical University, Beijing 100044, China
Hong-Fen Li, Department of Clinical Test, Hospital of Kailuan Coal mine, Tangshan 063000, Hebei Province, China
Wen-Geng Zhang, Institute of Biophysics, Chinese Academy of Science, Beijing 100044, China
Guo-Dong Cao, male, born on 1968-07-29 in Inner Mongolia, graduated from Beijing Medical University as a Ph.D in 1998, majorins molecular biology and biochemistry, having 9 papers published.
Author contributions: All authors contributed equally to the work.
Supported by the Chinese national scientific foundation, No.39 470316
Correspondence to: Dr. Guo-Dong Cao, Central Laboratory, People’s Hospital, Beijing Medical University, Beijing 100044, China
Received: June 10, 1998 Revised: July 4, 1998 Accepted: August 1, 1998 Published online: October 15, 1998
Abstract
AIM: To demonstrate whether bcl-2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC-803.
METHODS: To transduct a retrovirus containing bcl-2 antise nse RNA to MGC-803 cells and then to analyse the Bcl-2 protein expression in t he cells by Western blotting. To observe the morphology alteration, detect the G1 phase arrest by FCM, inhibition of proliferation by MTT method and tumorigenicity in nude mice.
RESULTS: The MGC-anti-bcl-2 cells shows contact inhibition, morphological alteration, from round or near round to shuttle like, decelerated growth rate, G1 phase arrest and weakened tumorigenicity in nude mice unlike the control (MGC-neo cells).
CONCLUSION: Our data show that antisense RNA to bcl-2, not only can induce apoptosis, but also reverse the biological behavior of MGC-803 cells. This would be a potential application to the gene therapy for stomach cancers.
